Exploration of the mechanism of Huangjing in the treatment of Alzheimer's disease based on network pharmacology
In order to explore the potential mechanism of action of Huangjing in treating Alzheimer's disease from the perspec-tive of multi-component,multi-target and multi-pathway,the active components and the corresponding targets of Huangjing were retrieved from TCMSP database,and related targets of Alzheimer's disease were retrieved from GeneCards database using"Alzheimer's disease"as the keyword.The intersection targets were obtained by mapping the two types of targets retrieved to each other.A Protein-Protein Interaction(PPI)network with intersection targets was constructed through the STRING platform.The core targets in PPI network were selected according to degree values and were visualized in Cytoscape3.8.The GO and KEGG enrichment analyses were performed for intersection targets using David 6.8 database.Finally,Schrodinger software was used to verify the molecular docking between the core targets and the key active ingredients.The results showed that:① Three key active components including Baicalein,β-sitosterol and Liquiritigenin were screened from Huangjing.Three core targets of Alzheimer's disease,namely AKT1,CASP3 and TP53 were selected from GeneCards database;② GO and KEGG enrichment analysis showed that the targets were mainly concentrated in cancer pathway,apoptosis and PI3K-Akt signaling pathway;③ According to the docking score of molecular docking,it can be showed that Baicalin and Liquiritigenin had better docking effect with AKT1 and TP53.These results indicated that Baicalein and Liquiritigenin were more likely to target AKT1 and TP53,and they can achieve anti-Alzheimer's effect by regulating apoptosis and P13K-Akt signaling pathway.
HuangjingAlzheimer's diseasenetwork pharmacologymechanism of actionmolecular docking
国家科技期刊平台
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