Effects of chrysotile on expression of Wnt5a,p16 and p21 in endothelial cells
Objective To investigate the influence of chrysotile on human umbilical vein endothelial cells(HUVECs).Methods HUVECs in the experimental group were treated with 50,100 and 200 mg/L chrysotile for 24,48 and 72 h.The control group was cultured with RPMI 1640 medium only.Morphological changes of HUVECs were observed.Cell viability was detected by MTT assay.β-Galactosidase staining was used to analyze the cell senescence.The cell survival rate was determined by tetramethylazolium blue assay.Wnt5a,p16 and p21 expression were investigated by qRT-PCR.Results Cells of the experimental group were oval and irregular,and some of them were round or irregular,with naked nuclei and vacuolar,and dead cells were present.With the increase of chrysotile concentration and exposure time,the level of cell viability decreased and the number of aged cells increased gradually(P＜0.05).The cell growth of HUVECs was more active when 100 mg/L chrysotile treated for 24 h.Compared with the control group,the mRNA expression levels of Wnt5a,p16 and p21 were increased in the experimental group(P＜0.05).Conclusion Chrysotile can promote HUVECs senescence,and Wnt5a,p16 and p21 are involved in this mechanism.
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