Effects of miR-107 on proliferation,invasion and migration of CAL27 cells in oral squamous cell carcinoma
Objective To investigate the expression miR-107 in oral squamous cell carcinoma CAL27 cell line and its effect on the proliferation,apoptosis,invasion and migration of CAL27 cells.Methods The expression of miR-107 CAL27 cell line and human oral epithelial HOEC cells were detected by real-time quantitative fluorescent PCR(qRT-PCR).MiR-107 mimic and miR-107 NC plasmid were transfected into CAL27 cells using liposome transfection method,and cells were divided into two groups,the miR-107 NC group used as a control group and the miR-107 mimic group used as a miR-107-overexpressed group.MTT cell proliferation assay was used to detect cell proliferation.Flow cytometry was used to detect cell apoptosis.Transwell chamber assay was used to detect cell migration and invasion.Results The expression of miR-107 was lower in CAL27 cell line than that in HOEC cells.OD values of the miR-107 mimic group were lower than those of the miR-107 NC group at the time of 24,48 and 72 h after transfection.The proportion of apoptotic cells was higher in the miR-107 mimic group than that of the miR-107 NC group,while the number of invasion and migration cells was lower than that of the miR-107 NC group(P＜0.05).Conclusion Up-regulation of miR-107 can inhibit proliferation,invasion and migration of CAL27 cells.
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