Endometritis in dairy cows is one of the major diseases causing huge economic losses in the dairy industry.Recent studies have shown that forsythoside A,extracted from the traditional Chinese herb forsythia,has a wide range of biological activities.The aim of this study was to elucidate the inhibitory effect of forsythoside A on Escherichia coli endotoxin mediated inflammatory damage in bo-vine endometrial epithelial cells(bEECs)as well as the possible molecular mechanisms in vitro.The results showed that conotoxin sig-nificantly attenuated the damage effect of bEECs in a model of LPS-induced inflammatory injury in bEECs.Results of qPCR showed that forsythoside A significantly inhibited the release of pro-inflammatory cytokines(TNF-α,IL-6 and IL-1β)in a dose-dependent manner.Results from immunofluorescence and immunoblotting confirmed that coniferin significantly inhibited the expression of high mobility protein(HMGB1),which in turn blocked the activation of nuclear factor NF-κB.In conclusion,the findings suggest that forsythoside A protects against LPS-induced inflammatory injury in bEECs by negatively regulating HMGB1 expression,mediated by the NF-κB pathway.
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