新疆驼乳源金黄色葡萄球菌MLST分型与毒力基因检测
MLST and virulence gene detection of Staphylococcus aureus isolated from camel raw milk in Xinjiang
吴自豪 1蔡依龙 2池昊明 2陈伟2
作者信息
- 1. 塔里木大学生命科学与技术学院,新疆 阿拉尔 843300
- 2. 塔里木大学动物科学与技术学院/新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,新疆 阿拉尔 843300
- 折叠
摘要
为了解驼乳源金黄色葡萄球菌多位点序列分型(multilocus sequence typing,MLST)与毒力基因携带情况,本试验对新疆驼乳源分离得到的金黄色葡萄球菌进行MLST、药物敏感性检测、生物被膜形成能力和溶血性检测以及毒力基因检测.结果表明,金黄色葡萄球菌在健康生驼乳中的分离率为5%(4/80);4 株金黄色葡萄球菌分别属于ST8114、ST8119、ST2073 和ST130(其中ST8114 和ST8119 为新的序列型),对 12 种抗菌药物全部敏感;在 4 株驼乳源金黄色葡萄球菌中可检测到sei、hla、hlb、clfA和clfB等毒力基因,并且均具有生物被膜形成能力和溶血活性.说明新疆驼乳源金黄色葡萄球菌分型丰富,在宿主致病和食品生产安全等方面存在隐患.本研究可为新疆驼乳源金黄色葡萄球菌的相关研究提供基础试验数据.
Abstract
To understand the multilocus sequence typing(MLST)and virulence gene of Staphylococcus aureus(S.aureus)from cam-el milk in Xinjiang,the MLST method,antibiotic susceptibility testing,biofilm-forming ability and hemolysis assessment,as well as virulence gene analysis were performed on the S.aureus isolated from camel milk samples in this study.The results showed that S.aureus was isolated from apparently healthy raw camel milk at a rate of 5%(4/80).All S.aureus isolates belonging to ST8114,ST8119,ST2073,and ST130(ST8114 and ST8119 are novel sequence types),respectively,and were sensitive to 12 antimicrobial antibiotics.The 4 isolates carried sei,hla,hlb,clfA,and clfB virulence genes and exhibited biofilm-forming ability and hemolytic ac-tivity.This result illustrates that S.aureus from camel milk is typologically rich and poses a risk to both host pathogenicity and food production safety.This study provides primary experimental data for the related research on S.aureus from Xinjiang camel milk.
关键词
驼乳/金黄色葡萄球菌/MLST分型/毒力基因/生物被膜Key words
camel milk/Staphylococcus aureus/MLST/virulence gene/biofilm引用本文复制引用
基金项目
新疆生产建设兵团重点领域科技攻关计划(2020AB025)
出版年
2024
国家科技期刊平台
NSTL
万方数据