Optimization of extraction technology of Isatis tinctoria L.var.praecox extractand its effect on mouse hair follicles
The aim of this experiment is to investigate the effects of the active ingredients in Isatis tinctoria L.var.praecox on the hair follicles of mice under different extraction conditions.The active ingredients in Isatis tinctoria L.var.praecox were extracted by ultrason-ic-assisted extraction using distilled water or anhydrous ethanol as solvents,respectively,and the optimal extraction conditions were al-so screened by response surface methodology.The extracts under different conditions were applied to the skin surface of depilated mice,and the differences in hair growth of mice in the blank control group,aqueous extraction group and alcohol extraction group were ob-served and analysed,and the differences in the density of hair follicles in the skin of the three groups of mice were observed by HE stai-ning,while apoptosis of hair follicle cells of the three groups was detected by TUNEL immunofluorescence method.The results showed that the active ingredient of Isatis tinctoria L.var.praecox extracted by anhydrous ethanol could inhibit the apoptosis of hair follicle cells,and the active ingredient of Isatis tinctoria L.var.praecox extracted by distilled water could induce the hair follicles to enter into the anagen phase earlier,and the active ingredients of Isatis tinctoria L.var.praecox extracted by the two methods could promote the growth of hair follicles.In conclusion,Isatis tinctoria L.var.praecox extract can promote the growth and development of hair follicles and inhibit the apoptosis of hair follicle cells.
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