In order to investigate the microbial diversity,and the antibacterial activity of the dominant microbes in situ concentration in soil,clindamycin was used as a selecting expressure for the enrichment in situ,and the microbial community diversity in soil was analyzed by using Illumina Navaseq high-throughput sequencing technology and culture-free methods.Meanwhile,resistant actinomycetes were screened out using microbial cultivation techniques,combined with the filter paper method and cake confrontation method.The findings showed that at the culture-free level,with the increased addition of clindamycin in the in soil,the species abundance decreased,while the dominant genera survived.In the concentration of 1∶10 and 1∶100 clindamycin in situ enrichment of soil microorganisms,the main dominant bacteria were Micrococcales,Frankiales,Corynebacteriales,Kineosporiales,and Streptomycetaies.The culturable level,a total of 207 actinomycetes were isolated from the clindamycin-enriched soil in situ,belonging to 44 species,6 genera,6 families,6 orders.Moreover,Streptomyces were the dominant genera,and 25 actinomycetes with excellent activity against Alternaria gaisen were selected.In conclusion,the clindamycin-enriched soil in situ is more conducive to the isolation of activity strains that antagonize pathogenic fungi,has a great prospects in the biological control of pathogens.
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