Analysis of diversity and antibacterial activity of soil microbial communities enriched with clindamycin in soil
In order to investigate the microbial diversity,and the antibacterial activity of the dominant microbes in situ concentration in soil,clindamycin was used as a selecting expressure for the enrichment in situ,and the microbial community diversity in soil was analyzed by using Illumina Navaseq high-throughput sequencing technology and culture-free methods.Meanwhile,resistant actinomycetes were screened out using microbial cultivation techniques,combined with the filter paper method and cake confrontation method.The findings showed that at the culture-free level,with the increased addition of clindamycin in the in soil,the species abundance decreased,while the dominant genera survived.In the concentration of 1∶10 and 1∶100 clindamycin in situ enrichment of soil microorganisms,the main dominant bacteria were Micrococcales,Frankiales,Corynebacteriales,Kineosporiales,and Streptomycetaies.The culturable level,a total of 207 actinomycetes were isolated from the clindamycin-enriched soil in situ,belonging to 44 species,6 genera,6 families,6 orders.Moreover,Streptomyces were the dominant genera,and 25 actinomycetes with excellent activity against Alternaria gaisen were selected.In conclusion,the clindamycin-enriched soil in situ is more conducive to the isolation of activity strains that antagonize pathogenic fungi,has a great prospects in the biological control of pathogens.
万方数据
维普
