Comparison of alcoholic liver disease model in C57BL/6J mice and Kunming mice using NIAAA method
To compare the effectiveness of constructing alcoholic liver disease models in C57BL/6J mice and Kunming mice,and analyze the subsequent changes in liver biochemical indicators,this experiment aims to establish a foundational basis for researching the treatment of alcoholic liver disease.A total of 36 female C57BL/6J mice and 36 female Kunming mice were randomly assigned to the control group(liquid chow+maltodextrin),the 16-day modeling group and the 19-day modeling group(liquid chow+alcohol,n=12).The NIAAA method was utilized to induce alcoholic liver disease in the mouse models.Results indicated that in the 16-day and 19-day modeling groups of C57BL/6J mice as well as the 16-day modelling group of Kunming mice,there were significant increases in serum ALT,AST,liver MDA,and TG levels compared to their respective control groups(P<0.05).Conversely,levels of serum ALB and liver SOD were significantly lower in the modeling groups compared to controls(P<0.05).Moreover,liver GSH level and liver coefficient in the 16-day and 19-day modeling groups of C57BL/6J mice were significantly higher than those in the control group(P<0.05).However,in the 19-day modeling group of Kunming mice,except for a lower liver SOD level compared to the control group(P<0.05),there were no other significant differences in indicators.Pathological examination of liver tissue sections revealed disordered arrangement of liver cells,hepatocyte necrosis,inflammatory infiltration and lipid droplets in the 16-day modeling groups of C57BL/6J mice and Kunming mice compared with the control groups.Observation of pathological sections showed that both strains of mice in the 16-day modeling group exhibited disordered arrangement of liver cells,hepatocyte necrosis,inflammatory infiltration and the presence of lipid droplets compared with the control groups.While the liver tissue of C57BL/6J mice in the 19-day modeling group still displayed pathological changes,the liver cells of Kunming mice in the 19-day modelling group had gradually returned to normal arrangement.C57BL/6J mice exhibited more severe alcohol-induced liver oxidative stress damage compared to Kunming mice,resulting in a more stable alcoholic liver model.The experimental results suggest that C57BL/6J mice are more susceptible to alcohol-induced liver oxidative stress damage than Kunming mice,thereby indicating a more stable alcoholic liver model.
万方数据
维普
