In order to investigate the hypoglycemic effect of Morus nigra L.leaves polysaccharide(MLP)combined with traditional hypoglycemic drug insulin(INS),chitosan(CTS)and MLP were used as raw materials,sodium tripolyphosphate(TPP)was used as crosslinking agent,and INS was used as model drug.Insulin-loaded chitosan-Morus nigra L.leaves polysaccharide(INS@CTS-MLP)nanoparticles were successfully prepared by ion crosslinking method.The results showed that the particle size of INS@CTS-MLP nanoparticles was(464.61±2.28)nm,the Zeta potential was(16.95±1.59)mV,the encapsulation efficiency was(61.63±4.54)%,and the drug loading was(12.22±1.23)%.In vitro release test showed that INS@CTS-MLP nanoparticles could effectively slow down the release of insulin in an acidic environment with pH=1.2.In vivo hypoglycemic activity test showed that INS@CTS-MLP nanoparticles had significant difference compared with MLP and INS@CTS nanoparticles at 4 weeks of administration(P<0.05).The blood glucose level of the MLP group was 60.07%of that of the model group(P<0.05),the blood glucose level of the INS@CTS nanoparticle group was 53.91%of that of the model group(P<0.05),and the blood glucose level of the INS@CTS-MLP nanoparticle group was only 40.23%of that of the model group(P<0.05),which proved that the combined use of MLP and INS had a significant synergistic hypoglycemic effect on diabetic mice.
关键词
药桑叶多糖/壳聚糖/药物载体/胰岛素/降血糖活性
Key words
Morus nigra L.leaves polysaccharide/chitosan/drug carrier/insulin/hypoglycemic activity
维普
万方数据