Preparation and hypoglycemic activity of INS@CTS-MLP nanoparticles
In order to investigate the hypoglycemic effect of Morus nigra L.leaves polysaccharide(MLP)combined with traditional hypoglycemic drug insulin(INS),chitosan(CTS)and MLP were used as raw materials,sodium tripolyphosphate(TPP)was used as crosslinking agent,and INS was used as model drug.Insulin-loaded chitosan-Morus nigra L.leaves polysaccharide(INS@CTS-MLP)nanoparticles were successfully prepared by ion crosslinking method.The results showed that the particle size of INS@CTS-MLP nanoparticles was(464.61±2.28)nm,the Zeta potential was(16.95±1.59)mV,the encapsulation efficiency was(61.63±4.54)%,and the drug loading was(12.22±1.23)%.In vitro release test showed that INS@CTS-MLP nanoparticles could effectively slow down the release of insulin in an acidic environment with pH=1.2.In vivo hypoglycemic activity test showed that INS@CTS-MLP nanoparticles had significant difference compared with MLP and INS@CTS nanoparticles at 4 weeks of administration(P<0.05).The blood glucose level of the MLP group was 60.07%of that of the model group(P<0.05),the blood glucose level of the INS@CTS nanoparticle group was 53.91%of that of the model group(P<0.05),and the blood glucose level of the INS@CTS-MLP nanoparticle group was only 40.23%of that of the model group(P<0.05),which proved that the combined use of MLP and INS had a significant synergistic hypoglycemic effect on diabetic mice.
Morus nigra L.leaves polysaccharidechitosandrug carrierinsulinhypoglycemic activity
万方数据
维普
