FOXM1c regulates the promoter activity of selective autophagy adaptor protein p62
To study the regulatory effects of transcription factor FOXM1c on the promoter activity of autophagy-related protein p62.Bioinformatics software was used to predict the binding sites of transcription factors FOXM1c and the promoter region of p62.The wild-type promoter fragment of p62 gene and the mutant p62 gene DNA fragment were synthesized,and the luciferase reporter gene vectors containing p62 wild-type and mutant promoter fragments were constructed respectively.The pcDNA3.1-FOXM1c-EGFP expression vector was constructed.The effect of FOXM1c on the transcriptional activity of the wild-type and the mutant p62 gene promoter was evaluated using the dual luciferase reporter gene detection system.The findings indicate that both the wild-type and mutant p62 promoters demonstrate transcriptional activity.Furthermore,the transcription factor FOXM1c was observed to have a negative effect on the wild-type p62 gene promoter,however,this negative effect became undetectable in the mutated-type p62 gene promoter.
万方数据
维普
