The preventive effect and mechanism of Populus euphratican alkaloids on acute gastric ulcer in rats
To explore the protective effect of Populus euphratican alkaloids on ethanol-induced acute gastric ulcers in rats,the superoxide dismutase(SOD)activity,malondialdehyde(MDA)content,epidermal growth factor(EGF),and vascular endothelial growth factor(VEGF)levels were observed.A total of 54 SD rats were randomly divided into six groups:a blank control group,a model group,a ranitidine group(75 g·L-1),and low-concentration,medium-concentration,and high-concentration of Populus euphratica alkaloid groups(300 μg·mL-1,600 μg·mL-1and 900 μg·mL-1,respectively).Following a 48-hour fast,each rat was administered the corresponding group of drugs by gavage at a dose of 10 mL·kg-1.After 1.5 hours,with the exception of the control group,95%ethanol(5 mL·kg-1)was administered orally to the remaining groups in order to create a model.Following a period of eight hours,the area of acute gastric ulcers in rats was measured.Histological examination using haematoxylin and eosin(HE)staining was then conducted to observe the pathological changes in gastric tissues within each group.The activity of SOD,the levels of MDA,VEGF and EGF in rat serum were examined.The results demonstrated that the gastric ulcer index of rats in the 600 μg·mL-1 Populus euphratica alkaloid group was significantly reduced in comparison to the model group.Additionally,the activity of SOD and the level of EGF were significantly increased,while the content of MDA and VEGF were significantly decreased.It can therefore be concluded that 600 μg·mL-1 Populus euphratica alkaloids can significantly alleviate ethanol-induced acute gastric mucosal injury and have a protective effect on ethanol-induced acute gastric ulcers in rats.
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