Objective To investigate the value of biochemical examination combined with electromyography in the di-agnosis of diabetic peripheral neuropathy.Methods The clinical data of 90 diabetic patients admitted to the Quan-zhou First Hospital from February 2020 to February 2022 were retrospectively selected,and the were divided into a group with peripheral neuropathy(n=40)and a group without peripheral neuropathy(n=50)according to whether they had peripheral neuropathy.Blood pressure,biochemical indexes and electromyography data were compared between the two groups of patients.Results The levels of cystatin C(Cys-C),uric acid,creatinine and glomerular filtration rate in the group with peripheral neuropathy were higher than those in the group without peripheral neuropathy,and the differences were statistically significant(all P<0.05).The left and right leg amplitude and right leg conduction veloc-ity of the peroneal sensory nerve in the group with peripheral neuropathy were lower than those in the group without peripheral neuropathy,and the latency of the left ankle of the motor nerve was longer than that in the group without pe-ripheral neuropathy,and the left ankle amplitude and left fibular head conduction velocity were lower than those in the group without peripheral neuropathy,and the latency of the left and right fibular head was longer than that in the group without peripheral neuropathy.The latency of left and right wrist of sensory nerve of median nerve were longer than that of group without peripheral neuropathy,and the latency of left and right wrist and the conduction velocity of left and right wrist were lower than those of group without peripheral neuropathy,and the latency of left and right wrist of motor nerve of median nerve were longer than those of group without peripheral neuropathy,and the fluctuation of left elbow,the conduction velocity of right elbow were lower than that of group without peripheral neuropathy,and the differences were statistically significant(all P<0.05).Conclusion Biochemical examination combined with electromy-ography has high clinical value in the treatment of diabetic peripheral neuropathy.