蛭龙活血通瘀胶囊对糖尿病心肌病小鼠心功能的影响
Effect of Zhilong Huoxue Tongyu Capsule on Cardiac Function of Diabetic Cardiomyopathy Mice
杨方 1刘平 1陈皓灵 1巫丹 1董丽1
作者信息
- 1. 西南医科大学附属中医医院心内科,四川泸州 646000
- 折叠
摘要
目的 探讨蛭龙活血通瘀胶囊(Zhilong Huoxue Tongyu capsule,ZL)对糖尿病心肌病(Diabetic cardiomy-opathy,DCM)小鼠心功能的影响.方法 本研究选取2023年2月1日开始于西南医科大学动物实验中心进行实验的27只C57BL/6J小鼠作为研究对象,按随机数表法分为对照组(n=9),实验组(n=18).实验组小鼠采用链脲佐菌素(Streptozocin,STZ)结合高脂饮食喂养,建立DCM小鼠模型.建模成功后,实验组分为模型组,蛭龙活血通瘀胶囊组,每组8只.蛭龙活血通瘀胶囊组给予ZL 6.24 g/(kg·d)灌胃,其余组给予等体积生理盐水,连续6周.观察3组小鼠心脏质量指数、血脂、心肌组织病理、心脏彩超.结果 给药6周后,蛭龙活血通瘀胶囊组小鼠心脏质量指数低于模型组,各项血脂指标及心功能指标优于模型组,差异有统计学意义(P均<0.05).H&E染色结果显示,蛭龙活血通瘀胶囊组小鼠心肌间质胶原纤维数量较模型组显著减少.结论 ZL具有抑制DCM小鼠心肌纤维化的作用,从而发挥心脏保护作用.
Abstract
Objective To investigate the effect of Zhilong Huoxue Tongyu capsule(ZL)on the cardiac function of dia-betic cardiomyopathy(DCM)mice.Methods This study selected 27 C57BL/6J mice from the Animal Experimental Center of Southwest Medical University on February 1,2023 as research objects,and were divided into control group(n=9)and experimental group(n=18)according to random number table method.The experimental group of mice are fed with Streptozocin(STZ)combined with a high-fat diet to establish a DCM model.After the successful modeling,the experimental group was divided into model group,Zhulong Huoxue Tongyu capsule group,8 animals in each group.The Zhilong Huoxuetongyu capsule group was given ZL 6.24 g/(kg·d)by gavage,and the other groups were given equal volume normal saline for 6 weeks.The heart mass index,blood lipid,cardiac tissue pathology and cardiac color ultrasound were observed in 3 groups.Results After 6 weeks of ZL treatment,the heart mass index of Zhilong Huoxue Tongyu capsule group was lower than that of the model group,and the blood lipid indicators and cardiac func-tion indicators were better than those of the model group,the differences were statistically significant(all P<0.05).H&E staining showed that the number of collagen fibers in the myocardial space of Bdellosaurus was significantly re-duced compared with the model group.Conclusion ZL can inhibit myocardial fibrosis in DCM mice and thus play a cardioprotective role.
关键词
蛭龙活血通瘀胶囊/糖尿病心肌病/心肌纤维化/心脏保护Key words
Zhilong Huoxue Tongyu capsule/Diabetic cardiomyopathy/Myocardial fibrosis/Cardiac protection引用本文复制引用
基金项目
四川省科技厅重点研发项目(2022YFS0618)
出版年
2024
NETL
NSTL
维普
万方数据