Objective To analyze the accuracy of serum C-peptide combined with blood glucose detection in the diag-nosis of diabetes.Methods A total of one hundred patients with diabetes who were treated in Fuzhou Second Hospital from May 2021 to May 2022 were selected and included in the analysis group,and one hundred and seven healthy physical examination subjects were selected at the same period to be included in the control group.They were jointly diagnosed of diabetes.The laboratory indicators,serum C-peptide,blood glucose test,combined diagnosis results were analysed.Results Serum C-peptide in analysis group was lower than that in control group,fasting blood glucose,2 h postprandial blood glucose and glycated hemoglobin levels were significantly higher than those in control group,and the difference was statistically significant(all P<0.05).Serum C-peptide test showed 102 positive cases and 105 negative cases,82 of which were diabetic patients.HbA1c was detected in 102 positive cases and 105 negative cases,80 of which were diabetic patients.A total of 102 positive cases and 105 negative cases were detected by the com-bined test,100 of which were diabetic patients.The diagnostic specificity,sensitivity and accuracy of diabetes pa-tients under the combined detection method were significantly higher than those under the single detection method,and the difference was statistically significant(all P<0.05).Conclusion The combination of serum C-peptide and blood glucose detection in the diagnosis of diabetes is conducive to the improvement of diagnostic accuracy and diag-nostic efficiency.