Objective To analyze the clinical significance of the combined test of serum C-peptide and glycosylated hemoglobin A1c for clinical diabetes.Methods A total of seventy patients with suspected diabetes admitted to Zibo Hospital of Traditional Chinese Medicine from February 2022 to June 2023 were selected as the study objects.Single serum C-peptide,glycosylated hemoglobin A1c and combined test were performed for the patients.Oral glucose toler-ance test was used as the gold standard to compare the diagnostic results.Results Among the 70 suspected patients,60 cases were confirmed positive by oral glucose tolerance test,and the accuracy and sensitivity of combined test were higher than those of single serum C-peptide and glycosylated hemoglobin A1c detection,and the differences were sta-tistically significant(both P<0.05).The specificity of combined test of cornbined test was not statistically significant compared with that of single serum C-peptide and glycosylated hemoglobin A1c(P>0.05).The positive predictive val-ues and negative predictive values of combined test were higher than those of single serum C-peptide and glycosyl-ated hemoglobin A1c,and the differences were statistically significant(both P<0.05).Conclusion The combined test of serum C-peptide and glycosylated hemoglobin A1c can improve the diagnostic accuracy of diabetes.