Objective To study the value of chemiluminescence immunoassay in the diagnosis of diabetes.Methods A total of 276 patients with suspected diabetes who were admitted to Juye Country North City Hospital from January to December 2023 were selected as the research objects.All patients were diagnosed by biochemical immunoassay.The results of oral glucose tolerance test were used as the gold standard to analyze the diagnostic efficacy of chemical lumi-nescence immunoassay.Results Oral glucose tolerance test detected 231 cases of positive,45 cases of negative.The accuracy,sensitivity,specificity,misdiagnosis rate,missed diagnosis rate,positive predictive value,negative predic-tive value of chemiluminescence immunoassay were 97.83%(270/276),98.27%(227/231),95.56%(43/45),4.44%(2/45),1.73%(4/231),99.13%(227/229),91.49%(43/47),and the kappa value was 0.921.Conclusion In the biochemi-cal immunoassay of diabetes,the use of chemiluminescence immunoassay has a high diagnostic value.