Co-incorporation of Chinese milk vetch(Astragalus sinicus L.)and chemical fertilizers alters microbial functional genes supporting short-time scale positive nitrogen priming effects in paddy soils

扫码查看

原文链接

NETL
NSTL
万方数据

外文摘要：Nitrogen(N)priming is a microbially mediated biochemical process as affected by different incorporation practices.However,little information is known about the microbial mechanisms driving the response of N priming to co-operation of Chinese milk vetch(CMV,Astragalus sinicus L.)and different rates of chemical fertilizers in paddy soils in South China.Here,an anaerobic incubation experiment was conducted to study N priming effects(PE)and their relationships with soil microbial functional genes after CMV incorporation alone(M),co-incorporation of CMV with 100％(normal dosage)chemical fertilizers(MC100),and co-incorporation of CMV with 80％chemical fertilizers(MC80).Co-incorporation of CMV and chemical fertilizers enhanced the short-time scale(the first 20 d of incubation)positive PE of N,while no significant differences existed among the three treatments on day 60 or 90 of incubation(P＞0.05).Compared with the M treatment,gross priming effect(GPE)in the MC100 and MC80 treatments significantly increased by 34.0％and 31.3％,respectively,and net priming effect(NPE)increased by 47.7％and 47.8％,respectively,during the first 20 d of incubation(P＜0.05).This was likely attributed to soil nutrient availability and added substrate quality.The MC100 and MC80 treatments increased the gdhA gene abundance by 5.0％and 9.8％,increased the gdh2 gene abundance by 12.7％and 45.7％,and increased the nasB gene abundance by 9.5％and 41.4％,respectively,in comparison with the M treatment on day 20 of incubation.Correlation analyses indicated that soil microbial functional genes involved in N mineralization(gdhA and gdh2),assimilatory nitrate reduction(nasB),and nitrification(amoB)were significantly correlated with N priming under different incorporation practices during the incubation period(P＜0.05).Thus,co-incorporation of CMV and chemical fertilizers can regulate soil microbial community functional gene structure,which may accelerate mineralization and assimilatory nitrate reduction and inhibit nitrification,thereby increasing the short-term positive PE of N in the present study.

外文关键词：

green manuregross priming effectincorporation practicemicrobial mechanismnet priming effectrice field soil

作者：

Limin WANG、Chunmei HE、Dongfeng HUANG、Juhua YU、Cailing LIU、Qinghua LI、Yibin HUANG、Juan LI、Fei WANG

展开 >

作者单位：

Institute of Resources,Environment and Soil Fertilizer,Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350012(China)

School of Resources and Environment Engineering,Anhui University,Hefei 230601(China)

基金：

Youth Talent Program of Fujian Academy of Agricultural Sciences,ChinaSpecial Fund of Fundamental Scientific Research at Nonprofit Research Institutions in Fujian,ChinaNatural Science Foundation of Fujian Province,ChinaModern Agro-Industry Technology Research System-Green Manure,China"5511"Collaborative Innovation Engineering,ChinaExternal Cooperation Program of Fujian Academy of Agricultural Sciences,ChinaFoundation of Fujian Academy of Agricultural Sciences,China

项目编号：

YC20190062022R10250012020J011358CARS-22-G-01XTCXGC2021009DWHZ-2022-20CXTD2021012-2

出版年：

2024

DOI：

10.1016/j.pedsph.2023.04.002

土壤圈(英文版)

中国土壤学会,中科院南京土壤研究所和土壤与农业可持续发展国家重点实验室

土壤圈(英文版)

CSTPCD

影响因子：0.477

ISSN：1002-0160

年,卷(期)：2024.34(3)