Study on Removal Simulation of Nitrogen and Phosphorus in Water Polluted with Runoff from Farm Land by a Psychrotolerant Bacterium
The biological enhanced removal of nitrate and dissolved phosphate in the mixture of natural water with runoff from farm land by a denitrifying bacterium Acinetobacter johnsonii DBP-3 was studied in a simulation system.The results showed that the added strain could remove strongly the nitrated and dissolved phosphate in water samples under the cultivation conditions of 10 ℃,and aeration in dark.After 8 days of cultivation,the concentrations of nitrate in sterile and raw water samples significantly decreased 78.5% and 70.2%,respectively;the dissolved phosphate significantly decreased by 82.4% and 74.6%,compared to the controlled treatment of without the strain.The strain showed high adaptability to the simulation system and kept numerical superiority during the whole experimental period.Compared to the temperature of 10 ℃,the cultivation temperature of 25 ℃ significantly increased the ability of strain DBP-3 to removal nitrate and dissolved phosphate and at 5 ℃ the ability decreased significantly;whereas,the strain DBP-3 still kept the metabolic activity even at 5 ℃.The ability of strain DBP-3 to remove nitrate and dissolved phosphate was not inhibited until the salinity reached 10%,indicative of the strong resistance to salinity.Carbendazim and chlorpyrifos significantly inhibited the removal of nitrate and dissolved phosphate at the concentrations of 80.0,60.0 mg/L,respectively,suggesting that the strain was tolerant to the two kinds of pesticide.It is indicated that the strain DBP-3 would have promising future in the treatment of eutrophic water body because of its strong ability to remove nitrate and dissolved phosphate and tolerance to salinity and pesticide.
runoffremoval of nitrogen and phosphorus at low temperaturecarbendazimchlorpyrifos
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