The Effects of Different Ratios of Dicyandiamide on Influence of Urease/Nitrification Inhibitor on Urea Hydrolysis
To explore the regulation effects of different ratios of dicyandiamide (DCD) on influence of urease/ nitrification inhibitor on urea nitrogen transformation,a 112-day indoor thermostatic incubation experiment was carried out to evaluate effects of combined application of urease inhibitor,boric acid (B),and nitrification inhibitor,dicyandiamide (DCD),on nitrogen loss.Four ratios of DCD application (2%,5%,8% and 13%,w/w) were established.The results showed that urease/nitrification inhibitor could effectively slow down the hydrolysis of urea.In 8% and 13% DCD treatments,the release peak of urea was postponed 7 days.The concentration of NH4+-N maintained at a high level from 14 d to the end of the incubation experiment,ranging from 148.918 to 187.105 mg/kg,which significantly limited the transformation of NH4+-N to NO3--N and decreased production of nitrate.At the end of the experiment,the concentration of NO3-N was in the range of 5.181 to 35.463 mg/kg.The combined application of boric acid and DCD was effective for more than 84 d in maintaining N in NH4 + form and reducing concentration of NO3--N.These results suggested that in 8% DCD treatment,the concentration of NH4+ reached the highest level and remained stable,which have the greatest potential to reduce nitrogen loss.