Effect of Salt Stress on Carbon and Nitrogen of Microbial Biomass and Activity of Enzyme in Greenhouse Soil
An indoor thermostatic incubation experiment was carried out to investigate the effect of salt stress on microbial biomass carbon and nitrogen content and enzyme activity of yellow cinnamon soil in Hefei area.In this experiment,a control treatment (CK) and 4 salt stress treatments with different salt contents (0.3％,0.6％,0.9％ and 1.2％) were set up,which were incubated for 75 d under 25 ℃ in greenhouse.Soil samples were collected separately on the 15th,30th,45th,60th and 75th day of incubation and then analyzed.The results showed that with the increasing salt concentration,soil microbial biomass C and N content decreased,and the negative effects of salt stress was the most significant on the 45th day of incubation.Treatment D (salt content:1.2％) reduced soil microbial biomass C and N by 65.62％ and 57.68％,respectively,compared to CK treatment.Salt stress had significant inhibitory effect on soil enzyme activity,with the activities of enzymes decreasing with the increasing salt content of experimental soil.The inhibitory effect of salt stress was more significant on urease,protease,alkaline phosphatase and cellulose.On the 75th day of incubation,treatment D (salt content:1.2％) reduced cellulose activity by 62.20％,compared to CK treatment.Correlation analysis indicated that soil microbial biomass C and N were highly linear with the activities of soil enzymes at the same level of salt stress.
salt stressmicrobial biomass carbonmicrobial biomass nitrogenenzymesoil
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