Characters of Solubilizing Phosphate,Secreting IAA and Organic Acids of Phosphorus-Solubilizing Bacteria from Rhizosphere of Lotus corniculatus
Phosphate solubilization property of phosphobacteria strains,isolated from rhizosphere of Lotus corniculatus,was determined by using phosphate-solubilizing zone(PKO inorganic culture medium and Mengjinna organic culture medium) and phospho-molybdate blue color methods.Of the 20 isolated strains,11 strains possessed higher phosphate-dissolving capacity,with the range of dissolving phosphate from 94.35 to 400.49 μg mL-1,among which strain LC15 showed the highest phosphate solubilization capacity.The further experimental results showed that there was no significance relativity between phosphate solubilization capacity and the total quantity of organic acids in strain fermentation broth or the pH value,but most of the 11 strains had the ability to produce IAA,among them stain LC20 had the maximum IAA-producing capacity of 42.39 μg mL-1,and the capacity of secreting organic acids of strain LC15 was 30.67mmol L-1,just below that of the stain LC14,which was 32.34 mmol L-1.In terms of all the properties of dissolving phosphate and secreting IAA and organic acids,strains LC15,LC16,LC19,LC20 and LC25 could be used as prospective strains for Lotus corniculatus inoculant.
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万方数据
维普
