Effects of Acid Treatments on Soil Bacterial Community Structure and Diversity in the Subtropical Granite Area
Acid deposition significantly influenced ecological system structure and function,but the research on the effect of acid deposition on soil bacteria community and diversity was less.This study used granite soil profiles (0-100cm) suffered serious acid deposition in the subtropical area as research objectives.The batch leaching experiments had been carried with different pH solutions (HCl-NH4Cl,pH =3.5,4.5,5.5) for soil from A profile (0-12 cm),B profile (12-50 cm) and C profile (50-100 cm).Then the variability of soil bacterial community structure and diversity were discussed before and after acid treatments.The 16S rRNA genes of soil bacterial were analyzed by the MiSeq High-throughput Sequencing Technique.The results showed that the HCl-NH4Cl acid solution significantly reduced bacterial diversity at soil A,B and C layers,and soil bacterial diversity suffered the biggest drops at soil pH of 3.5,and the least drops at soil pH of 4.5 treated with acid treatment.The HCl-NH4Cl acid solution significantly influenced soil bacterial structure and changed soil bacterial dominant population.The dominant population was changed from Acidobacteria to Proteobacteria at soil A layer,and from Proteobacteria to Actinobacteria at soil B and C layers.The change in bacterial relative abundance and the principal component analysis showed that there was no significant variation of soil bacterial community among pH 3.5,pH 4.5 and pH 5.5,but soil bacterial community structure at different soil layers had the same responses to acid solution,which caused by the difference in soil physical and chemical properties among different acid treatments.The significant effects of acid solution on soil bacterial community and dominant population were closely related with soil propriety,and counteracted soil evolution and ecosystem succession.
HCl-NH4Cl acid solutionGraniteSoil profileBacterial community structureDiversity
NETL
NSTL
万方数据
维普
