Effects of Saline-alkali Treatment on Rhizosphere Soil Microbial Community and Network Complexity of Tall Fescue
[Objective]Tall fescue(Festuca elata'crossfire Ⅱ')was used as the test material to study the effects of different levels of saline-alkali treatment on the diversity,composition and network complexity of bacterial and fungal communities in the rhizosphere soil of tall fescue.[Method]Four salts(NaCl,Na2SO4,NaHCO3,and Na2CO3)were mixed in a 9∶1∶1∶9 M ratio,and four treatments of 0(S0),200(S1),400(S2)and 600 mmol/L(S3)were set.Illumina MiSeq high-throughput sequencing was used to measure soil microbial community.[Result]S,and S2 saline-alkali treatments had no significant effect on soil fungal diversity and network complexity compared with S0 treatment,while S3 treatment significantly reduced soil fungal diversity and network complexity.Saline-alkali treatment also shifted fungal community composition,with increased relative abundances of dominant phyla Ascomycota and decreased relative abundances of Chytridiomycota.Compared with fungal community,saline-alkali treatment had less effect on soil bacterial community.S3 saline-alkali treatment reduced the complexity of soil bacterial network,but had no significant effect on bacterial diversity and community composition.SEM showed that the saline-alkali effects on soil fungal community composition were primarily mediated by soil organic carbon and soil available phosphorus).[Conclusion]High concentration saline-alkali treatment reduced the diversity and network complexity of fungi in rhizosphere soil of tall fescue,and changed the composition of fungal community,but had little effect on bacterial community.
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