盐碱处理对高羊茅根际土壤微生物多样性和网络复杂性的影响
Effects of Saline-alkali Treatment on Rhizosphere Soil Microbial Community and Network Complexity of Tall Fescue
唐慧敏 1倪晓臻 1王颖超 1张雅洁 1刘慧1
作者信息
- 1. 德州学院生命科学学院,山东德州 253023
- 折叠
摘要
[目的]以高羊茅(Festuca elata'Crossfire Ⅱ')为试材,研究不同程度盐碱处理对高羊茅根际土壤细菌和真菌群落多样性、组成和网络复杂性的影响.[方法]按9∶1∶1∶9摩尔比例混合NaCl、Na2SO4、NaHCO3和Na2CO3四种盐,配制0、200、400和600 mmol L-1 4个浓度盐溶液,分别为S0、S1、S2和S3处理.不同浓度盐溶液定时定量浇入种植高羊茅盆栽土壤中,90天后采用高通量测序技术检测土壤微生物群落.[结果]与S0处理相比,S1和S2盐碱处理对土壤真菌多样性和网络复杂性无显著影响,S3处理显著降低了土壤真菌多样性和网络复杂性.盐碱处理也改变了土壤真菌群落组成,随盐碱处理程度的增加,优势门子囊菌门真菌相对多度逐渐增加,壶菌门真菌相对多度逐渐降低.与真菌相比,盐碱处理对土壤细菌群落影响较小,仅S3处理降低了土壤细菌网络复杂性,但对细菌多样性和群落组成无显著影响.结构方程模型结果显示,盐碱处理通过影响有机碳和有效磷对土壤真菌群落产生间接影响.[结论]高浓度盐碱处理降低了高羊茅根际土壤真菌多样性和网络复杂性,改变了真菌群落组成,但对细菌群落影响较小.
Abstract
[Objective]Tall fescue(Festuca elata'crossfire Ⅱ')was used as the test material to study the effects of different levels of saline-alkali treatment on the diversity,composition and network complexity of bacterial and fungal communities in the rhizosphere soil of tall fescue.[Method]Four salts(NaCl,Na2SO4,NaHCO3,and Na2CO3)were mixed in a 9∶1∶1∶9 M ratio,and four treatments of 0(S0),200(S1),400(S2)and 600 mmol/L(S3)were set.Illumina MiSeq high-throughput sequencing was used to measure soil microbial community.[Result]S,and S2 saline-alkali treatments had no significant effect on soil fungal diversity and network complexity compared with S0 treatment,while S3 treatment significantly reduced soil fungal diversity and network complexity.Saline-alkali treatment also shifted fungal community composition,with increased relative abundances of dominant phyla Ascomycota and decreased relative abundances of Chytridiomycota.Compared with fungal community,saline-alkali treatment had less effect on soil bacterial community.S3 saline-alkali treatment reduced the complexity of soil bacterial network,but had no significant effect on bacterial diversity and community composition.SEM showed that the saline-alkali effects on soil fungal community composition were primarily mediated by soil organic carbon and soil available phosphorus).[Conclusion]High concentration saline-alkali treatment reduced the diversity and network complexity of fungi in rhizosphere soil of tall fescue,and changed the composition of fungal community,but had little effect on bacterial community.
关键词
盐碱处理/细菌群落/真菌群落/土壤理化性质/高通量测序Key words
Saline-alkali treatment/Fungal community/Bacterial community/Soil physicochemical property/High-throughput sequencing引用本文复制引用
基金项目
国家自然科学基金(32001103)
德州学院科学研究基金(2019xjrc317)
德州学院横向科研项目(HXKT2023070)
山东省省属普通本科高等学校教师访学研修项目()
出版年
2024
NETL
NSTL
万方数据