湖南省油茶良种根际微生物区系分析
Analysis of Inter-root Microbiota of Improved Camellia oleifera Varieties in Hunan Province
董文统 1田诗义 1叶佳伟 1潘忠飞 1袁德义 1周道顺 2刘慧敏 2邹锋1
作者信息
- 1. 中南林业科技大学林学院经济林培育与保护教育部重点实验室,经济林育种与栽培国家林草局重点实验室,湖南 长沙 410004
- 2. 中国林业科学研究院经济林研究所,河南 郑州 450003
- 折叠
摘要
[目的]为了弄清湖南省油茶主栽良种根际微生物区系特征,筛选出关键差异微生物菌群,为油茶林土壤生物菌肥研发提供参考.[方法]利用高通量测序技术和 OTU分析等方法,对湖南省油茶良种'华硕'(HS)、'华鑫'(HX)、'湘林 210'(XL210)以及普通油茶(CK)根际土壤微生物多样性及差异进行了分析,同时测定了油茶根际土壤理化性质及酶活性,运用冗余分析方法,探讨土壤因子与根际微生物之间的关系,并进行核心菌群的筛选.[结果]①油茶良种根际微生物数量的排序均为HS＞HX＞XL210＞CK.铁、铜、总有机碳、铵态氮、β-葡萄糖苷酶是影响土壤细菌多样性的主要因子,全钾、电导率、植酸酶是影响土壤真菌多样性的主要因子.②油茶良种与对照存在显著性差异的微生物为Microscillaceae、那提普斯壳科Natipusillaceae、Leotiomycetes.[结论]差异微生物可作为人工合成菌群的核心候选成员,为油茶林土壤生物菌肥的研发奠定了基础.
Abstract
[Objective]The aims were to figure out the inter-root microbiota characteristics of Camellia oleifera varieties and screen out the key differential microflora in Hunan Province,which could provide an information for the development of soil biofertilizers in C.oleifera forests.[Methods]High throughput sequencing technology and OTU analysis were used to characterize the inter-root microbiota of C.oleifera varieties'Huashuo'(HS),'Huaxin'(HX),'Xianglin 210'(XL210),and CK.The physicochemical and enzyme activity properties of C.oleifera inter-root soils were also determined.The relationship between soil factors and inter-root microorganisms was investigated by redundancy analysis(RDA)and screened out the core flora in C.oleifera.[Results]① The order of the number of inter-root microorganisms in C.oleifera varieties was HS＞HX＞XL210＞CK.Fe,Cu,TOC,ammonium nitrogen,and β-glucosidase were the main factors affecting the diversity of soil bacteria.Total potassium,electrical conductivity,and phytase were the main factors affecting the diversity of soil fungi.② Microorganisms with significant differences among different C.oleifera varieties were Microscillaceae,Natipusillaceae,unclassified_c_Leotiomycetes.[Conclusion]These differential microorganisms could be used as the core candidate members,and laid an foundation for development of the soil biofungus fertilizer in C.oleifera forests.
关键词
油茶/良种/根际微生物/关键微生物区系Key words
Camellia oleifera/Improved varieties/Rhizosphere microorganism/Key flora microorganism引用本文复制引用
基金项目
国家重点研发计划项目(2022YFD2200400)
国家重点研发计划项目(2020YFD1000700)
出版年
2024
NETL
NSTL
万方数据