Analysis of Inter-root Microbiota of Improved Camellia oleifera Varieties in Hunan Province
[Objective]The aims were to figure out the inter-root microbiota characteristics of Camellia oleifera varieties and screen out the key differential microflora in Hunan Province,which could provide an information for the development of soil biofertilizers in C.oleifera forests.[Methods]High throughput sequencing technology and OTU analysis were used to characterize the inter-root microbiota of C.oleifera varieties'Huashuo'(HS),'Huaxin'(HX),'Xianglin 210'(XL210),and CK.The physicochemical and enzyme activity properties of C.oleifera inter-root soils were also determined.The relationship between soil factors and inter-root microorganisms was investigated by redundancy analysis(RDA)and screened out the core flora in C.oleifera.[Results]① The order of the number of inter-root microorganisms in C.oleifera varieties was HS＞HX＞XL210＞CK.Fe,Cu,TOC,ammonium nitrogen,and β-glucosidase were the main factors affecting the diversity of soil bacteria.Total potassium,electrical conductivity,and phytase were the main factors affecting the diversity of soil fungi.② Microorganisms with significant differences among different C.oleifera varieties were Microscillaceae,Natipusillaceae,unclassified_c_Leotiomycetes.[Conclusion]These differential microorganisms could be used as the core candidate members,and laid an foundation for development of the soil biofungus fertilizer in C.oleifera forests.
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