Spatial Distribution of Enzyme Activities in the Rhizosphere of Celosia argentea Linn.in Cd-contaminated Soil Using in-situ Soil Zymography
[Objective]The aim was to investigate the influence of soil cadmium(Cd)contamination on the spatial distribution of β-glucosidase and acid phosphatase in the rhizosphere of Cd hyperaccumulator Celosia argentea Linn.[Method]The in-situ soil zymography was used to obtain the distribution of β-glucosidase and acid phosphatase in the rhizosphere of C.argentea in Cd-polluted soil and non-polluted soil.[Result]The Results showed that the hot spots of β-glucosidase and acid phosphatase were along entire root of C.argentea,which were relatively higher at the root tips.In both the polluted and non-polluted soils,the grey value of β-glucosidase and acid phosphatase at the root center were significantly higher than those 2 mm away from the root center.In Cd-polluted soil,the grey value of β-glucosidase and acid phosphatase in the root center decreased by 5%and 3%when compared to that in the non-polluted soil.[Conclusion]The results showed that the spatial distribution of soil enzyme activities was closely related to the root distribution.Soil Cd contamination had a significant negative influence on the soil enzyme activities.
Soil zymographyCelosia argentea Linn.CadmiumSpatial distribution of the enzyme activity
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