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稻田土壤中病毒多样性研究

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病毒是地球上最丰富多样的微生物类群,广泛存在于各种生物体及环境中,但关于稻田土壤的病毒组研究较少.使用公共数据库中的稻田土壤宏基因组测序数据,构建由8 791个病毒操作分类单元(viral operational taxonomic units,vOTU)和168 940个蛋白序列组成的稻田土壤病毒数据库(Paddy soil virus database,PSVD),该数据库中31.41%的序列能够预测出物种分类地位信息,这些病毒序列分属于76个已知的病毒科,以有尾病毒纲(Caudoviricetes)病毒为主,宿主分布在11个细菌门中.同时还对稻田土壤病毒中所携带的辅助代谢基因(Auxiliary Metabolic Genes,AMGs)进行分析,发现了 39个可能广泛存在与稻田系统的碳水化合物活性酶(Carbohydrate active enzymes,CAZymes),该类基因的存在,可能对生物地球化学循环产生潜在的影响.综上,本研究创新性构建了PSVD数据库(https://github.com/lzk98/PSVD),并利用DRAM-v挖掘了稻田土壤系统中的 AMGs 基因,为后期稻田土壤病毒组的研究奠定了研究基础,并为稻田病毒群落的解析提供新的见解.
Virus Diversity Studies in Paddy Soils
[Objective]Viruses are the most abundant and diverse group of microorganisms on Earth as they can be found in a wide range of organisms and environments.Despite their ubiquity,there are relatively few studies on the virome in paddy soil systems,which is an area that warrants investigation.[Method]In this study,virus sequences were mined from rice soil macrogenome sequencing data in public databases and characterized.[Result]A database of paddy soil viruses(PSVD)was constructed,which contained 8 791 viral operational taxonomic units(vOTUs)and 168 940 protein sequences.In PSVD,31.41%of the sequences could predict the taxonomic status of the species.These viral sequences belonged to 76 known virus families,with the majority of viruses coming from the Caudoviricetes,and their hosts were mainly distributed across 11 bacterial phyla.Also,Auxiliary Metabolic Genes(AMGs)carried by rice field soil viruses were analyzed,and 39 carbohydrate-active enzymes(CAZymes)genes,which may have been widely distributed in rice soil systems,were identified,and the presence of these genes contributed to biogeochemical cycling.[Conclusion]In summary,this study innovatively constructed a PSVD database(https://github.com/lzk98/PSVD)and used DRAM-v to mine AMG genes in paddy soil systems,thus,laying a research foundation for later studies of paddy soil virus groups and providing new insights for the resolution of paddy virus communities.

ViromicSoilRice fieldCarbohydrate-active enzymes

刘泽锟、黄士轩、杨美艳

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华南农业大学食品学院,广东省食品质量安全重点实验室,广州 510642

华南农业大学农学院,广州 510642

病毒组学 土壤 稻田 碳水化合物活性酶

2024

土壤学报
中国土壤学会

土壤学报

CSTPCD北大核心
影响因子:2
ISSN:0564-3929
年,卷(期):2024.61(6)
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