Subject librarians serve as the backbone force in knowledge services within academic libraries,and with the advent of the AIGC era,their AI literacy significantly influences the prospects of AIGC application in academic libraries as well as the efficiency enhancement of subject services.This study starts from the perspective of AI literacy among subject librarians,and investigates the current status and the influencing factors of AI literacy of subject librarians through questionnaire surveys.Based on these findings,strategies for improving the AI literacy of subject librarians are discussed from four levels:policy,society,library,and librarian,aiming to provide references for innovation and transformation of libraries in the era of AIGC.