Nutritional composition analysis of sea cucumber ovum and the effect of extracts on estradiol secretion in ovarian cancer cells
Sea cucumber ovum is the gonad of female sea cucumber ovum,which is often treated as the waste in sea cucumber processing,but it contains rich nutrients and a variety of active factors.In this study,the composition and content of general nutrients,elements and amino acids of sea cucumber ovum were analyzed.At the same time,the effects of sea cucumber ovum water extract and alcohol extract on the proliferation of human ovarian cancer OVCAR-3 cells and its estradiol secretion were studied.Results showed that the water content of sea cucumber o-vum was 86.63％,and the contents of crude protein,crude lipid and crude polysaccharide(dry weight)were 65.06％,14.82％and 1.93％,respectively.A total of 17 amino acids were detected,and the total amino acids con-tent was 519.09 mg/kg.Among them,the content of essential amino acids was 197.04 mg/kg.Sea cucumber ovum contains a variety of elements beneficial to human body.Sea cucumber ovum extract significantly inhibited the pro-liferation of human ovarian cancer OVCAR-3 cells,and the contents of estradiol in the supernatant of cell culture solution were increased by water extract and alcohol extract in sea cucumber ovum,and the content of estradiol in supernatant can be significantly increased by alcohol extract(P＜0.01).The above results show that sea cucumber ovum is rich in nutrition,and its extract has a significant role in promoting the secretion and regulation of estradiol in human ovarian cancer OVCAR-3 cells,so it has high exploitation and utilization values.
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