Deep-sea microorganisms play an important role in organic matter mineralization.The ultimate fate and microbial impact of recalcitrant dissolved organic carbon(RDOC)in the deep sea still need to be further re-searched.In this study,microbes of water samples at different depths(200-4 700 m)of 6 stations in the western Pacific Ocean were enriched with humic acid and landfill leachate at low temperature for one year,respectively.Bacterial diversity was analyzed using 16S rRNA gene high-throughput sequencing technology.Results showed that the bacterial compositions of enriched samples with different substrates were similar.The dominant genera contained Alcanivorax,Methylophaga and Polycyclovorans of Gammaproteobacteria,Thalassospira and Paracoccus of Alpha-proteobacteria and Nocardioides of Actinobacteria.The dominant bacteria may participate in the deep mineralization of RDOC in situ.
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