Study on the Impact of Anxiety Levels on Voluntary Exercise Motivation in Rats under Chronic Restraint Stress
OBJECTIVE:This study aimed to explore the relationship between different levels of anxiety and voluntary exercise willingness in rats using a chronic restraint stress anxiety model.METHODS:Twenty-four male 6-week-old SD rats were divided into a control group(C group,n=8)and a model group(Mgroup,n=16).The model group was subjected to 4 weeks of chronic restraint stress to establish the anxiety model.The elevated plus maze and sucrose preference test were used to evaluate the successful establishment of the animal model.Based on the behavioral test results,the model rats were further divided into a mild anxiety group(M1 group,n=8)and a severe anxiety group(M2 group,n=8).The C group was kept undisturbed for 4 weeks.The voluntary wheel-running system was used to monitor the rats'voluntary exercise amount within 24 hours.RESULTS:(1)Compared to the C group,the Mgroup rats showed a significant decrease in total distance traveled,number of entries into the open arms,and distance traveled in the open arms in the elevated plus maze.(2)Compared to the C group,both the M1 and M2 groups showed significant decreases in total distance traveled(P＜0.01),number of entries into the open arms(P＜0.01),and distance traveled in the open arms(P＜0.05)in the elevated plus maze.Additionally,the M2 group showed significant decreases in these parameters compared to the M1 group(P＜0.05 for total distance,P＜0.01 for number of entries,and P＜0.01 for distance traveled in the open arms).(3)Compared to the C group,both the M1 and M2 groups showed a significant decrease in sucrose preference rate(P＜0.05),and the M2 group showed a significant decrease compared to the M1 group(P＜0.05).(4)Compared to the C group,both the M1 and M2 groups showed a significant reduction in voluntary wheel-running activity(P＜0.05),and the M2 group showed a significant decrease compared to the M1 group(P＜0.05).CONCLUSION:There is a correlation between the level of anxiety and voluntary exercise willingness in rats subjected to chronic restraint stress,with more severe anxiety leading to lower voluntary exercise willingness.
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