Differences in Plasma Metabolites among MS Risk Factors with Different CRF Levels
This study aims to analyze plasma differential metabolites related to metabolic syndrome(MS)risk factors across different cardiorespiratory fitness(CRF)levels,and to explore the metabolomic mechanisms through which variations in CRF levels influence MS risk factors,providing a theoretical basis for improving metabolic health in populations at risk of MS through CRF enhancement.Methods:Ninety subjects aged 40～65 were selected and categorized into low(L),moderate(M),and high(H)CRF levels using tertile classification.Plasma samples were analyzed using untargeted metabolomics based on high-performance liquid chromatography coupled with mass spectrometry(LC-MS).Data were preprocessed using IsoMS Pro 14.1 and SPSS 26.0 software.Three-tiered metabolite identification methods were employed for structural identification of me-tabolites.Multivariate statistical analysis was conducted using SIMCA-P 14.1 software following data format conversion.Dif-ferential metabolites were identified and characterized using volcano plot analysis and variable importance in projection(VIP)values.Metabolic pathway analysis was performed using MetaboAnalyst 5.0 software,with pathway annotations based on the KEGG database.Results:(1)Eight differential metabolites were identified between the H and L groups,each exhibiting specif-ic trends:increased levels of alpha-ketoglutarate(↑),succinic acid(↑),arginine(↑),gamma-aminobutyric acid(↑),serine(↑),and decreased levels of valine(↓),methionine(↓),and glutamine(↓).(2)Metabolic pathway analysis re-vealed that CRF primarily influences arginine biosynthesis,tricarboxylic acid(TCA)cycle,and cysteine and methionine me-tabolism,among 11 biological pathways.Conclusion:This study identifies eight metabolites as potential biomarkers distinguis-hing MS risk factors among individuals with different CRF levels.Furthermore,elucidation of pathways such as arginine bio-synthesis and TCA cycle highlights the mechanisms through which CRF impacts MS risk factors,suggesting that high CRF lev-els may act protectively against MS risk factors.
万方数据
维普
