CONVERSION OF SUGARCANE BAGASSE TO SUGARS AND ETHANOL BY MIXED CULTURE SOLID-STATE FERMENTATION
The effects of fermentation and direct conversion of ethanol were investigated by single and mixed culture of Trichoderma reesei CICC40359 and Penicillium decumbens LSM-1 with solid-state fermentation (SSF) of alkali-pretreated sugarcane bagasse.Fungi biomass,total sugars concentration,enzyme activity and ethanol yield were investigated in the SSF process.The results indicated that mixed culture effect was much better than pure culture.The highest total sugars concentration 20.21 g/L and reducing sugars concentration 12.47 g/L were obtained in mixed culture at 30 ℃,inoculation amount 8％ after 72 h culturing,β-glucosidase activity and fungi biomass produced were 0.48 IU/mL and 0.21 g/g DM respectively at 144 h.The maximum ethanol concentration 5.83 g/L (40.84％ of the theoretical yield) was achieved in the first 24 h when inoculating Saccharomyces cerevisiae with simultaneous saccharification and fermentation of the 3 days SSF substrate mixtures including sugars,crude unprocessed enzymes and the remained solids.These findings suggest a simplified crude enzymes process at the site of ethanol production using a common lignocellulosic substrate may be employed in lieu of commercial enzyme preparations.
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万方数据
维普
