SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE
Lcc1 gene from Trametes trogii CICC 2689 was integrated into Saccharomyces cerevisiae Y6 by Di-CRISPR to achieve stable expression of laccase,and to investigate the stress resistance of laccase on phenolic inhibitors and simultaneous saccharification and fermentation.The results showed that the recombinant strains Y6/Lac in the medium containing inhibitors had shorter lag phase,higher exponential phase growth rate and final biomass than Y6.At the same time,the degradation rate of alkali lignin by Y6/Lac increased by 41%,and the production efficiency of ethanol by simultaneous saccharification and fermentation increased by 14%.
recombinant Saccharomyces cerevisiaelaccaseDi-CRISPRsimultaneous saccharification and fermentationcellulosic ethanol
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