表达漆酶的重组酿酒酵母菌株同步糖化发酵产乙醇特性研究
SIMULTANEOUS SACCHARIFICATION AND CELLULOSIC ETHANOL FERMENTATION OF RECOMBINANT YEAST STRAIN WITH EXPRESSING LACCASE
李娟娟 1王志鹏 1朱士论 1叶禹彤 1路佳琪 1田沈1
作者信息
- 1. 首都师范大学生命科学学院,北京 100048
- 折叠
摘要
将来源于硬毛拴菌(Trametes trogii CICC 2689)的lcc1基因通过Di-CRISPR技术整合到酿酒酵母Y6中以实现漆酶的稳定表达,考察漆酶在酚类抑制剂及同步糖化发酵中的抗逆作用.结果显示:平板拮抗和液体摇瓶实验中,重组菌株Y6/Lac的生长状况、对数期生长速率及延滞期较对照菌株Y6均表现出相应优势;同时Y6/Lac对碱木质素的降解率提高41%,同步糖化发酵产乙醇效率提高14%.
Abstract
Lcc1 gene from Trametes trogii CICC 2689 was integrated into Saccharomyces cerevisiae Y6 by Di-CRISPR to achieve stable expression of laccase,and to investigate the stress resistance of laccase on phenolic inhibitors and simultaneous saccharification and fermentation.The results showed that the recombinant strains Y6/Lac in the medium containing inhibitors had shorter lag phase,higher exponential phase growth rate and final biomass than Y6.At the same time,the degradation rate of alkali lignin by Y6/Lac increased by 41%,and the production efficiency of ethanol by simultaneous saccharification and fermentation increased by 14%.
关键词
重组酿酒酵母/漆酶/Di-CRISPR/同步糖化发酵/纤维素乙醇Key words
recombinant Saccharomyces cerevisiae/laccase/Di-CRISPR/simultaneous saccharification and fermentation/cellulosic ethanol引用本文复制引用
基金项目
国家自然科学基金面上项目(31971202)
国家科技支撑计划(2019YFB1503802)
出版年
2024
NETL
NSTL
万方数据