The Response Surface Method Optimizes the Process of Extracting Alkaloids from Lotus Leaves
Based on the acid leaching method and the single-factor experimental method,this experiment optimizes the lotus leaf alkaloid extraction process by combining the method of ultrasonic auxiliary extraction and Box-Behnken response surface analysis,and uses the lotus leaf alkali yield as an indicator to investigate the effect of ultrasonic time(min),ultrasonic power(W),ultrasonic tem-perature(℃),hydrochloric acid mass fraction(％)on the yield of lotus leaf alkali,and obtains the opti-mal extraction condition and maximum yield of lotus leaf base.The test shows that:through Variance analysis method,the resulting rate change is the most affected by ultrasonic time and hydrochloric acid mass fraction,while the ultrasonic temperature is the smallest,the optimal extraction process condi-tions after optimization:when the ultrasonic time is 37 min,the ultrasonic power is 530 W,the ultra-sonic temperature is 48 degrees ℃,and the hydrochloric acid mass fraction is 0.56％,the optimal ex-traction rate of lotus base is(1.69±0.05)mg/g.
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