]In this experiment,yeast wild-type(BY4741)and HOG1 mutant(AHOG1)strains were used as materials to detect the changes of growth,apoptosis,intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential of yeast cells under zinc sulfate stress.The results showed that high concentration of zinc inhibits cell growth,causes the level of intracellular ROS increased,and induced apoptosis.At the concentration of 3 mmol/L zinc sulfate treatment group,zinc tolerance and mitochondrial membrane potential were lower,meanwhile,the intracellular ROS level and apoptosis rate were higher significantly in the yeast strain lacking HOG1(AHOG1)compared to the strain BY4741.These results indicated that HOG1 may regulate the zinc sulfate tolerance of yeast cells by controlling the generation of intracellular ROS.
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