miR-431-5p Promotes the Proliferation and Migration of Esophageal Squamous Cell Carcinoma Cells via the MAPK/ERK Pathway
To detect miR-431-5p expression in ESCC and explore its function,we analyzed the TCGA public data-base for miR-431-5p expression in esophageal cancer.RT-qPCR was used to detect the expression of miR-431-5p in ESCC tissues and cells.CCK-8 assay,cell scratch and transwell migration assay and Western blotting were performed to detect the effect of miR-431-5p on the proliferation,migration and invasion of ESCC cells suffering the transfection of miR-431-5p mimics and inhibitors.Our results showed that miR-431-5p was highly expressed in esophageal cancer.Af-ter transfection of miR-431-5p mimics,the proliferation rate,migration rate and invasion rate of KYSE30 cells were in-creased by 24.19%,30.86%and 50.29%(P<0.05);while the number and migration rate of TE-11 cells were decreased by 21.43%and 9.52%(P<0.05)after transfection of miR-431-5p inhibitors;miR-431-5p mimics increased the expression of p-ERK in KYSE30 cells by 11.68%;miR-431-5p inhibitor decreased the expression of p-ERK in TE-11 cells by 45.73%.In summary,miR-431-5p promotes the proliferation,migration and invasion of ESCC cells through the MAPK/ERK pathway,providing experimental basis for the development of effective targets for the treatment of ESCC.
miR-431-5pesophageal squamous cell carcinomacell proliferationinvasion and migrationMAPK/ERK
NETL
NSTL
万方数据
维普
