Isolation and identification of the pathogen causing anthracnose disease on Parrotia subaequalis
Parrotia subaequalis is a rare and endangered plant species endemic to China.Years of investigation have shown that this species is affected by anthracnose disease.Based on sample collection,pathogenic fungus isolation,and pathogenicity identification,this study employed morphological and molecular biological methods to determine the type of pathogenic fungus causing P.subaequalis anthracnose disease.The results showed that one strain of pathogenic fungus was isolated from diseased leaves and named YLMJ.This fungus can invade Parrotia subaequalis leaves,causing symptoms consistent with those observed in the wild.Morphological identification revealed that the pathogenic fungus grew radially on culture media,with initial white colonies turning into dark brown later.The hyphae were thin and filamentous with multiple sharp-angled branches,which was similar in appearance to Colletorichum acutatum.Sequence alignment results indicated that YLMJ had the highest homology with C.acutatum in internal transcribed spacer,actin gene and beta-tubu-lin gene sequences,and they clustered in the same clade on phylogenetic trees.Combining morphological and multi-gene identification results confirmed that the pathogenic fungus causing P.subaequalis anthracnose disease was C.acutatum.Isolation and identification of the pathogenic fungus causing P.subaequalis anthracnose disease laid the foundation for subsequent scientific prevention of the disease and research on its pathogenic mechanism.
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