黄秋葵多糖和黄酮的双水相萃取
Study on the aqueous two-phase extraction of polysaccharides and flavonoids from okra
郭晨忆 1李帆 1窦啸天 1张宜兴 1李佳雪 1祝子坪1
作者信息
- 1. 台州学院 药学院,浙江 台州 318000
- 折叠
摘要
为了从黄秋葵中分离出多糖和黄酮,采用(NH4)2SO4 和乙醇形成的双水相进行萃取,经单因素和正交试验优化萃取条件.结果表明:每 10 mL萃取液含 1.98 g(NH4)2SO4 和 3.9 mL乙醇,50℃条件下振荡萃取 15 min,黄秋葵多糖萃取率为(86.12±1.87)%(n=3),黄酮萃取率为(78.38±1.35)%(n=3).该方法可以一次性萃取出黄秋葵多糖和黄酮,且萃取率较高,为黄秋葵功能性成分的提取奠定了基础.
Abstract
To separate polysaccharides and flavonoids from okra,an aqueous two-phase composed of(NH4)2SO4 and ethanol was used for the extraction.The extraction conditions were optimized through single-factor and orthogonal tests.The results showed that under the conditions that for each 10 mL of the extract containing 1.98 g(NH4)2SO4 and 3.9 mL ethanol,extracted at 50 ℃ for 15 minutes,the extraction rate in okra was(86.12±1.87)%(n=3)for polysaccharide and was(78.38±1.35)%(n=3)for flavonoids.This method can extract okra polysaccharides and flavonoids at one time,with a high extraction rate,and can lay the foundation for the extraction of functional components of okra.
关键词
黄秋葵/多糖/黄酮/双水相萃取Key words
okra/polysaccharides/flavonoids/aqueous two-phase extraction引用本文复制引用
基金项目
国家级大学生创新创业训练计划(202310350068)
台州市科技计划(21nya18)
出版年
2024
NETL
NSTL
万方数据