Study on the aqueous two-phase extraction of polysaccharides and flavonoids from okra
To separate polysaccharides and flavonoids from okra,an aqueous two-phase composed of(NH4)2SO4 and ethanol was used for the extraction.The extraction conditions were optimized through single-factor and orthogonal tests.The results showed that under the conditions that for each 10 mL of the extract containing 1.98 g(NH4)2SO4 and 3.9 mL ethanol,extracted at 50 ℃ for 15 minutes,the extraction rate in okra was(86.12±1.87)%(n=3)for polysaccharide and was(78.38±1.35)%(n=3)for flavonoids.This method can extract okra polysaccharides and flavonoids at one time,with a high extraction rate,and can lay the foundation for the extraction of functional components of okra.
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