Objective This study was to investigate the expression levels of serum exosome miRNA-132 and silent information regulator1(SIRT1)in patients with type 2 diabetes mellitus(T2DM)and mild cognitive impairment(MCI),and their diagnostic value in the diagnosis of T2DM with MCI.Methods 60 patients with type 2 diabetes mellitus(T2DM)from the Endocrinology Department of Taizhou People's Hospital were selected,including 30 patients with normal cognition(T2DM+NCI)and 30 patients with mild cognitive impairment(T2DM+MCI).The expression levels of exosomal miRNA-132 and SIRT1 in the serum were detected.Results There were sig-nificant differences in gender,age,education level,and LPA between the T2DM+MCI group and the T2DM+NCI group(P＜0.05).Compared with the T2DM+NCI group,the expression levels of serum exosomal miR-NA-132 and SIRT1 were lower in the T2DM+MCI group,and the differences were statistically significant(P＜0.05).The ROC curve shows that when serum exosomal miRNA-132 is detected alone,its sensitivity is 0.933,its specificity is 0.5,and the area under the curve(AUC)is 0.727.When serum SIRT1 is detected alone,the sen-sitivity is 0.433,the specificity is 1,and the area under the curve(AUC)is 0.673.When the two are jointly de-tected,the sensitivity is 0.867,the specificity is 0.6,and the area under the curve(AUC)is 0.752.Conclusions Compared with the T2DM+NCI group,the expression levels of serum miRNA-132 and SIRT1 in the T2DM+MCI group were lower in the same direction.The expression of miRNA-132 in serum exosomes and serum SIRT1 are of certain significance in diagnosing T2DM with mild cognitive impairment,and the diagnostic value is higher when the two are jointly detected.
关键词
2型糖尿病/轻度认知障碍/外泌体miRNA-132/SIRT1
Key words
type 2 diabetes mellitus/mild cognitive impairment/exosome miRNA-132/SIRT1
维普
万方数据