Effect of celastrol on inflammatory injury in rats with acute liver failure by regula-ting HMGB1/TLR4/NF-κB signal pathway
Objective To analyze the effect of celastrol on inflammatory injury in rats with acute liver failure(ALF)by regulating HMGB1/TLR4/NF-κB signal pathway.Methods Rats were divided into normal group,model group,celastrol 12.5 mg/kg group,celastrol 50 mg/kg group,hepatocyte growth promoting factor group(2 mg/kg),celastrol+ glycyrrhizic acid group(50 mg/kg+20 mg/kg),the intervention lasted for 3 days.The general condition of rats was ob-served,the levels of serum liver function and inflammation indexes of rats were analyzed by automatic biochemical analy-zer.HE staining was used to observe the pathology of rat liver tissue.TUNEL staining was used to observe the apoptosis of rat hepatocytes,and Western blotting was used to observe the expression of HMGB1/TLR4/NF-κB pathway proteins in rat liver.Results Compared with the normal group,the mortality rate,levels of serum ALT,AST,TBil,apoptosis rate of liver cells,and the expression of HMGB1,TLR4,p-NF-κB/NF-κB in liver tissue of the model group increased(P<0.05).Compared with the model group,the mortality rate,levels of serum ALT,AST,TBil,apoptosis rate of liv-er cells,and the expression of HMGB1,TLR4,p-NF-κB/NF-κB in liver tissues of the celastrol 12.5 mg/kg and celas-trol 50 mg/kg groups and hepatocyte growth promoting factor group decreased(P<0.05).Compared with the celastrol 50 mg/kg group,the levels of serum ALT,AST,TBil,apoptosis rate of liver cells,and the expression of HMGB1,TLR4,p-NF-κB/NF-κB in liver tissues of rats in the celastrol + glycyrrhizic acid group decreased(P<0.05).Conclusion Celastrol may reduce liver inflammation and hepatocyte apoptosis,and protect liver tissue in ALF rats by inhibiting HMGB1/TLR4/NF-κB pathway.
CelastrolAcute liver failureInflammatory injuryHMGB1/TLR4/NF-κB signal pathway
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