The non-alcoholic fatty liver model was compared between high-fat diet and methio-nine choline deficiency diet based on bioinformatics
Objective To comprehensively compare the impact of high-fat diet(HFD)and methionine-choline-deficient(MCD)on the non-alcoholic fatty liver disease(NAFLD)model in C57 mice.Employing bioinformatics methods,to unveil differentially expressed genes(DEGs)and conduct functional analysis.Methods The expression data of GSE78170 and GSE123354 were obtained from GEO database.DEGs of C57 mice control group,MCD diet group and C57 mice control group and HFD diet group were compared using R language.Further differential analysis,functional enrichment analysis,gene and protein interaction network construction and overlapping DEGs comparative analysis were carried out.Results A total of 44 up-regulated genes and 16 down-regulated genes induced by HFD were screened out;43 up-regulated genes and 73 down-regulated genes were induced by MCD.The enrichment analysis of the top 12 biological processes suggested that HFD was associated with simple fat accumulation in liver,while MCD could promote the further transformation of NAFL into NASH in all aspects.Conclusion Genes induced by HFD predominantly relate to hepatic fat accumulation,while MCD tends to promote a comprehensive progression of NAFL towards NASH.
NETL
NSTL
万方数据
维普
