基于生物信息学对比分析高脂饮食和蛋氨酸胆碱缺乏饮食构建的非酒精性脂肪性肝病小鼠模型
The non-alcoholic fatty liver model was compared between high-fat diet and methio-nine choline deficiency diet based on bioinformatics
王熠昕 1谭诗云2
作者信息
- 1. 武汉大学第一临床学院,湖北 武汉 360060;武汉大学人民医院消化内科/消化系统疾病湖北省重点实验室
- 2. 武汉大学人民医院消化内科/消化系统疾病湖北省重点实验室
- 折叠
摘要
目的 比较高脂饮食(high-fat diet,HFD)饮食和蛋氨酸胆碱缺乏(methionine-choline-deficient,MCD)饮食对 C57 小鼠非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)模型的影响,通过生物信息学方法揭示其差异表达基因(GEO-data-based analysis of differentially expressed genes,DEGs)并进行功能分析.方法 从 GEO 数据库中获取 GSE78170、GSE123354 的表达数据.使用 R语言分别比较 C57 小鼠对照组与 MCD饮食组、C57 小鼠对照组与 HFD饮食组数据获得 DEGs.对 DEGs进行进一步的差异分析、功能富集分析、构建基因和蛋白互作网络及重叠 DEGs 对比分析.结果 筛选出 HFD 诱导的上调基因 44 个,下调基因 16个;MCD诱导的上调基因 43 个,下调基因 73 个;分析富集分析排名前 12 位的生物学过程,提示 HFD 与肝脏单纯脂肪积累相关,MCD则能全方位促进 NAFL进一步转变为 NASH.结论 HFD诱导的基因主要涉及肝脏脂肪积累,而 MCD 更倾向于全方位促进NAFL向 NASH的进展.
Abstract
Objective To comprehensively compare the impact of high-fat diet(HFD)and methionine-choline-deficient(MCD)on the non-alcoholic fatty liver disease(NAFLD)model in C57 mice.Employing bioinformatics methods,to unveil differentially expressed genes(DEGs)and conduct functional analysis.Methods The expression data of GSE78170 and GSE123354 were obtained from GEO database.DEGs of C57 mice control group,MCD diet group and C57 mice control group and HFD diet group were compared using R language.Further differential analysis,functional enrichment analysis,gene and protein interaction network construction and overlapping DEGs comparative analysis were carried out.Results A total of 44 up-regulated genes and 16 down-regulated genes induced by HFD were screened out;43 up-regulated genes and 73 down-regulated genes were induced by MCD.The enrichment analysis of the top 12 biological processes suggested that HFD was associated with simple fat accumulation in liver,while MCD could promote the further transformation of NAFL into NASH in all aspects.Conclusion Genes induced by HFD predominantly relate to hepatic fat accumulation,while MCD tends to promote a comprehensive progression of NAFL towards NASH.
关键词
非酒精性脂肪性肝病/蛋氨酸胆碱缺乏/高脂饮食/生物信息学分析Key words
Non-alcoholic fatty liver disease/Methionine-choline-deficient/High-fat diet/Bioinformatics analysis引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据