HMGB2在ESCC患者外周血T细胞中的表达与临床病理指标的关系
Expression of HMGB2 in peripheral blood T cells of ESCC patients and its relation-ship with clinicopathological parameters
彭仁佳 1马军 2安超 2银潇迪 3韩海燕 2王向鹏4
作者信息
- 1. 新乡医学院医学技术学院,河南 新乡 453003;郑州大学第二附属医院检验科
- 2. 郑州大学第二附属医院检验科
- 3. 郑州大学第二附属医院检验科;郑州大学第二附属医院肿瘤科
- 4. 新乡医学院医学技术学院,河南 新乡 453003
- 折叠
摘要
目的 研究 HMGB2 在 ESCC患者外周血 T细胞中的表达水平及与临床病理指标的关系.方法 以 31 例 ESCC 患者和13 名健康人为研究对象,通过流式细胞术、RT-qPCR和 Western blotting检测 HMGB2 在外周血 T细胞中的表达情况;根据肿瘤分期和组织分化程度分组,评估 HMGB2 与病理指标的关系.结果 与健康对照组相比,HMGB2 在 ESCC患者 CD4+和 CD8+T 细胞中高表达.肿瘤Ⅰ～Ⅱ期组和Ⅲ～Ⅳ期组,高、中分化组和低分化组 HMGB2 表达率均显著高于健康对照组.临床病理结果显示,HMGB2 的表达水平:低分化组高于高、中分化组;肿瘤组织>5 cm组高于肿瘤组织≤5 cm 组,浸润深度 T3～T4 组高于 T1～T2 组,临床 TNM 分期Ⅲ～Ⅳ期组高于Ⅰ～Ⅱ期组.结论 HMGB2 在 ESCC患者 T细胞中高表达,其表达与 ESCC 患者的临床 TNM 分期、组织学分化程度、肿瘤大小、浸润深度均呈正相关.HMGB2 可作为评价 ESCC患者预后的潜在生物标志物和治疗靶点.
Abstract
Objective To study the expression level and clinical significance of high mobility group protein B2(HMGB2)in peripheral blood T lymphocytes of patients with esophageal squamous cell carcinoma(ESCC).Methods The expression of HMGB2 in peripheral blood T lymphocytes was detected by flow cytometry,RT-qPCR and Western blotting in 31 ESCC patients and 13 healthy subjects.The relationship between HMGB2 and pathological indexes was evaluated according to tumor stage and tissue differentiation.Results Compared with healthy controls,HMGB2 was highly expressed in CD4+ and CD8+ T cells in ESCC patients.The expression rate of HMGB2 in tumor stage Ⅰ-Ⅱ and Ⅲ-Ⅳ groups,as well as high,middle differentiation and low differentiation groups were significantly higher than those in healthy control group.The clinicopathological analysis showed that the expression rate of HMGB2 was higher in the low differentiation group than that in the high,middle differentiation group;the group with tumor tissue>5 cm was high-er than that with tumor tissue≤5 cm;the infiltration depth of T3-T4 group was higher than that of T1-T2 group;the clinical TNM stage Ⅲ-Ⅳ group was higher than the stage Ⅰ-Ⅱ group.Conclusion HMGB2 is highly expressed in T lymphocyte subsets of ESCC patients,and its expression is positively correlated with clinical TNM stage,histological differentiation degree,tumor size and invasion depth of ESCC patients.HMGB2 can be used as a potential biomarker and therapeutic target to evaluate the prognosis of ESCC patients.
关键词
食管鳞状细胞癌/高迁移率族蛋白2/T细胞/临床病理参数Key words
Esophageal squamous cell carcinoma/High mobility group protein 2/T lymphocyte subsets/Clinico-pathological parameter引用本文复制引用
基金项目
河南省科技攻关项目(232102311062)
河南省卫健委医学科技攻关项目(SBGJ202102176)
河南省医学科技攻关计划联合共建项目(LHGJ20220491)
出版年
2024
NETL
NSTL
维普
万方数据