Effect of SOCS-3 and its gene polymorphism on esophageal mucosal injury in obese patients with gastroesophageal reflux disease
Objective To investigate the effect of SOCS-3 gene polymorphism on esophageal mucosal injury in obese people gastroesophageal reflux disease(GERD).Methods A total of 100 patients with GERD with more than 8 scores of Gerd Q scale were randomly selected.Based on PCR-sequencing,the genotyping of SNP polymorphic rs4969168 in SOCS-3 gene was studied.The correlation between obesity-related indexes(BMI,weight,height,waist circumfer-ence),GERD Los Angeles grade and its gene polymorphism were studied,and the expression of SOCS-3 was studied by Western blotting.Results The expression of SOCS-3 protein in obese GERD patients was the highest,the overweight group was the second,and the normal group was the lowest.The value of BMI in patients with different genotypes of GERD,AA:(26.21±3.41)kg/m2,AG:(24.42±2.04)kg/m2,GG:(23.90±2.68)kg/m2(P=0.011),and the waist circumference of patients with different genotypes of GERD,AA:(91.34±10.85)cm,AG:(88.90±8.93)cm,GG:(84.28±8.00)cm(P=0.03).The frequency of allele A at rs4969168 locus was positively correlated with the average level of BMI and waist circumference.The degree of esophageal mucosal injury in GERD patients was signifi-cantly associated with rs4969168 gene polymorphism(P=0.037).With the decrease of the frequency of allele A,the proportion of severe esophageal mucosal injury in GERD patients increased.Conclusion Allele A of rs4969168 plays a protective role in esophageal mucosal inflammatory injury in patients with GERD,and the increased expression of SOCS-3 protein in esophageal mucosa may be an important factor in the protective effect of allele A in patients with high BMI.
NETL
NSTL
万方数据
维普
