Cannabidiol regulates the polarization direction of macrophages in ConA-induced immune hepatitis in mice
Objective To investigate the effect of cannabidiol(CBD)on hepatic macrophages in mice with acute immune hepatitis induced by concanavalin A(ConA).Methods Thirty male C57BL/6 mice were randomly divided into normal control group,ConA model group and CBD experimental group(low dose 5 mg/kg,medium dose 15 mg/kg,high dose 30 mg/kg).After the experiment,the liver weight of mice was measured.ALT and AST levels were measured in peripheral blood,the contents of TNF-α,IL-6,TGF-β and IL-10 in liver were determined by ELISA,and the types and proportions of macrophages in liver were determined by flow cytometry.Results Compared with the normal control group,the liver weight of mice in ConA model group was decreased.The serum ALT and AST levels were increased(P＜0.01).The liver cytokines TNF-α and IL-6 levels were increased,the levels of TGF-β and IL-10 were decreased(P＜0.05).The proportion of M1-type macrophages was increased,while the proportion of M2-type macrophages was decreased(P＜0.05).Compared with the ConA model group,the liver weight of mice in the CBD experimental group increased to different degrees,and the serum ALT,AST levels decreased.The decrease was more significant in the medium and high dose groups(P＜0.01 and P＜0.05).Meanwhile,the levels of TNF-α,IL-6 in the liver of mice were decreased,and the levels of TGF-β,IL-10 were increased.The decrease of TNF-α,IL-6 and the increase of TGF-β in the middle and high dose groups were more significant(P＜0.05).In addition,the proportion of M1-type macrophages decreased and the proportion of M2-type macro-phages increased in the low-dose and medium-dose CBD groups,and the increase of M2-type macrophages was more signifi-cant in the medium-dose group(P＜0.05).Conclusion CBD can alleviate ConA-induced acute immune liver injury in mice by promoting the polarization of macrophages M1 to M2 in mice liver.
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