结直肠癌患者血清外泌体lnc-KIAA1586-2的表达及其临床意义
Expression and clinical significance of serum exosomal lnc-KIAA1586-2 in colorectal cancer
朱春凯 1李俊蒙 2郑培明 1李刚 1王荣1
作者信息
- 1. 河南省人民医院检验科,河南郑州 450003
- 2. 河南省人民医院胃肠外科,河南郑州 450003
- 折叠
摘要
目的 探讨血清外泌体来源lnc-KIAA1586-2在结直肠癌(colorectal cancer,CRC)患者中的表达水平及其临床意义.方法 收集2019年1月至2020年12月临床明确诊断的110例CRC患者及同期100名健康体检者血清标本,同时收集所有患者的临床资料.qRT-PCR法检测血清外泌体中lnc-KIAA1586-2的表达水平,电化学发光法检测血清CEA与CA19-9的表达水平.采用ROC曲线评价不同指标的诊断效能,并分析其与患者临床病理特征的关系.采用Cox比例风险模型进行多变量回归分析.结果 CRC患者血清外泌体中lnc-KIAA 1586-2的相对表达水平(2.96±0.13)显著高于健康对照组(1.56±0.11)(t=9.53,P＜0.001),患者术后血清外泌体lnc-KIAA1586-2相对表达水平显著下降.ROC曲线分析结果表明,血清外泌体lnc-KIAA1586-2诊断CRC的AUC为0.832,显著优于传统肿瘤标志物CEA、CA19-9的诊断效能.单因素分析发现血清外泌体lnc-KIAA1586-2相对表达量与肿瘤长径(x2=6.53,P＜0.05)、TNM分期(x2=5.61,P＜0.05)及淋巴结转移(x2=8.49,P＜0.05)等均有显著相关性.进一步多变量回归分析发现,手术前血清外泌体lnc-KIAA1586-2的相对表达水平(HR=2.858,95％CI:1.218～4.984,P=0.012)可以作为CRC患者预后生存的独立预测因子.结论 CRC患者血清外泌体lnc-KIAA1586-2相对高表达,具有良好的诊断效能,有望成为CRC患者诊断和预后评估的潜在生物标志物.
Abstract
Objective To investigate the expression and clinical significance of serum exosomal lnc-KIAA1586-2 in colorectal cancer(CRC)patients.Methods Peripheral serum samples from 110 CRC patients and 100 healthy sub-jects were collected from Jan.2019 to Dec.2020.General data and follow-up information of CRC patients were also col-lected.The expression levels of serum exosomal lnc-KIAA1586-2 were detected via qRT-PCR.The serum concentrations of traditional biomarker(CEA and CA19-9)were measured via a chemiluminescent detection system.ROC curve and AUC were used to estimate the diagnostic capacity,and the relationship with clinicopathological features of CRC patients was also investigated.Multivariate regression analysis was performed using Cox proportional hazard analysis.Results The expression level of serum exosomal lnc-KIAA1586-2 in CRC was significantly higher than that in healthy control group(t=9.53,P＜0.001),and the relative expression level decreased significantly after surgery.The area under the ROC curve of lnc-KIAA1586-2 was 0.832,which was higher than the diagnostic accuracies of CEA and CA19-9.Further analysis found that the expression level of exosomal lnc-KIAA1586-2 was significantly correlated with tumor size(x2=6.53,P＜0.05),TNM stage(x2=5.61,P＜0.05)and lymph node metastasis(x2=8.49,P＜0.05).Moreover,mult-ivariate analysis results indicated that exosomal lnc-KIAA1586-2 was an independent prognostic factor in CRC.Conclusion Our data demonstrated that exosomal lnc-KIAA1586-2 might be a promising noninvasive biomarker for the diagnosis and prognosis evaluation of CRC.
关键词
结直肠癌/外泌体/诊断/lnc-KIAA1586-2/生物标志物Key words
Colorectal cancer/Exosome/Diagnosis/lnc-KIAA1586-2/Biomarker引用本文复制引用
基金项目
国家自然科学基金青年基金(81802094)
河南省医学科技攻关计划(LHGJ20210017)
出版年
2024
NETL
NSTL
万方数据