甲基莲心碱对避水应激大鼠结肠运动的影响及其机制
The effect of Neferine on colonic motility induced by water avoidance stress in rats and its mechanism
夏源 1罗和生1
作者信息
- 1. 武汉大学人民医院消化内科,湖北 武汉 430060
- 折叠
摘要
目的 研究甲基莲心碱对避水应激大鼠结肠运动的影响和相关机制.方法 建立避水应激大鼠模型,记录造模期间每组大鼠每日平均粪粒数,通过 HE染色观察大鼠结肠病理改变.通过 RM6240 多通道生理信号系统记录造模大鼠体外结肠运动,以及不同浓度的甲基莲心碱对正常大鼠离体结肠肌条收缩活动的影响,通过 Western blotting评估大鼠结肠神经元一氧化氮合成酶蛋白(neuronal nitric oxide synthase,nNOS)的表达水平.结果 甲基莲心碱明显抑制避水应激所导致的大鼠结肠运动亢进,同时在体外水平以浓度依赖性的方式降低环形肌条和纵行肌条的收缩幅度.Western blotting结果显示,与对照组相比,给药组大鼠的 nNOS蛋白表达上调.结论 甲基莲心碱能抑制避水应激导致的大鼠结肠运动亢进,并且这种作用可能是通过一氧化氮途径实现的.
Abstract
Objective To preliminarily investigate the effects and potential mechanisms of Neferine on colonic mo-tility induced by water avoidance stress in rats.Methods A rat model of water avoidance stress was established,and the average daily number of fecal pellets per day in each group during the modeling period was recorded.Pathological changes in the colon of rats were observed by HE staining.The RM6240 multi-channel physiological signaling device was used to monitor in vitro colonic motility in model rats and to examine the impact of various Neferine concentrations on the contractile activity of isolated colonic muscle strips in normal rats.The expression of neuronal nitric oxide syn-thase(nNOS)in the rat colon was quantified by Western blotting.Results Neferine decreased the contraction ampli-tude of circular and longitudinal muscle strips in a concentration-dependent manner in vitro,significantly inhibiting the hypermotility of the colon in rats induced by water avoidance stress.According to Western blotting data,nNOS protein expression was elevated in the treated rats compared to the control group.Conclusion Neferine reverses colonic motility hyperactivity in water avoidance stress rats,and this action may be partially mediated by the nitric oxide(NO)pathway.
关键词
甲基莲心碱/避水应激/nNOS/结肠高动力Key words
Neferine/Water avoidance stress/nNOS/Colon hypermotivity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据