The effect of Neferine on colonic motility induced by water avoidance stress in rats and its mechanism
Objective To preliminarily investigate the effects and potential mechanisms of Neferine on colonic mo-tility induced by water avoidance stress in rats.Methods A rat model of water avoidance stress was established,and the average daily number of fecal pellets per day in each group during the modeling period was recorded.Pathological changes in the colon of rats were observed by HE staining.The RM6240 multi-channel physiological signaling device was used to monitor in vitro colonic motility in model rats and to examine the impact of various Neferine concentrations on the contractile activity of isolated colonic muscle strips in normal rats.The expression of neuronal nitric oxide syn-thase(nNOS)in the rat colon was quantified by Western blotting.Results Neferine decreased the contraction ampli-tude of circular and longitudinal muscle strips in a concentration-dependent manner in vitro,significantly inhibiting the hypermotility of the colon in rats induced by water avoidance stress.According to Western blotting data,nNOS protein expression was elevated in the treated rats compared to the control group.Conclusion Neferine reverses colonic motility hyperactivity in water avoidance stress rats,and this action may be partially mediated by the nitric oxide(NO)pathway.
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