Effect of RNF20 on DNA damage repair in ultraviolet ray-induced gastric cancer cells
Objective To investigate the effect of ring finger protein 20(RNF20)deficiency on DNA damage and repair in gastric cancer cells exposure to ultraviolet ray and its underlying mechanism.Methods MGC803 gastric canc-er cell lines with stable low expression of RNF20 were constructed by lentivirus vector,and they were divided into con-trol group and RNF20 deficient group.The cell proliferation was detected by CCK-8 method.The total irradiation dose of 20 J/m2 ultraviolet radiation was used to irradiate MGC803 gastric cancer cells.The levels of γ-H2AX,RAD51 and p21 proteins in the two groups were detected by Western blotting and immunofluorescence techniques.Results The ex-pression of green fluorescent protein was observed by fluorescence microscope in both groups.CCK-8 showed that de-creased expression of RNF20 promoted the proliferation of gastric cancer cells.Western blooting results showed that the expression of RNF20 protein in knockdown group was lower than that in control group.Compared with the control group,after 20 J/m2 ultraviolet irradiation,γ-H2AX disappeared more slowly,the expression of RAD51 protein decreased,and the expression of p21 protein decreased more slowly in the knockdown group.Conclusion RNF20 deficiency can inhibit the repair process of DNA damage induced by ultraviolet rays in gastric cancer cells.
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