槐花外泌体减轻低氧诱导的胃和小肠黏膜急性氧化应激损伤研究
Sophora japonica L.flower exosomes attenuate hypoxia-induced acute oxidative stress injury to the stomach and small intestine
张恒 1王德智 1廖星晨 1张明杰 2王梦琪 2许俊锋 3王昕 2金鹏 3盛剑秋2
作者信息
- 1. 解放军医学院,北京 100853
- 2. 中国人民解放军总医院第七医学中心消化内科
- 3. 中国人民解放军总医院第一医学中心消化内科医学部
- 折叠
摘要
目的 分析槐花外泌体在低氧诱导的胃和小肠黏膜急性氧化应激损伤中的作用.方法 通过超速离心法提取槐花外泌体并完成电镜和粒径鉴定,采用低压氧环境控制系统构建小鼠低氧急性胃肠损伤模型,在低氧第 1 天给予槐花外泌体灌胃,第 3 天安乐死小鼠,收集消化道组织进行 HE染色,收集胃和小肠黏膜进行 ROS检测.期间记录小鼠的进食量、体质量、粪便性状和便血情况.结果 口服槐花外泌体可改善低氧导致的小鼠血便、稀便和体质量下降,减轻低氧引起的胃和小肠黏膜损伤,降低 ROS 水平.结论 口服槐花外泌体可有效缓解低氧引起的胃和小肠黏膜急性氧化应激损伤.
Abstract
Objective To analyze the role of Sophora japonica L.flower exosomes in hypoxia-induced acute oxidative stress injury to the stomach and small intestine.Methods Sophora japonica L.flower exosomes were extracted using ultracentrifugation followed by characterization through transmission electron microscopy and particle size measurement.Hypoxia-induced acute gastric and intestinal injury was triggered using placing mice in a ventilated hypoxic chamber sys-tem for 3 days.PBS or Sophora japonica L.flower exosomes were orally administered on day 1 under hypoxia.Mice were euthanized on day 3.Food consumption,body weight,stool score and stool blood score were recorded daily.The gastro-intestinal tract was isolated for HE staining.The gastric and small intestinal mucosa was collected for ROS detection.Results Sophora japonica L.flower exosomes treatment ameliorated acute gastric and small intestinal mucosal injury and downregulated ROS induced by hypoxia.Conclusion Oral administration of Sophora japonica L.flower exosomes effectively attenuated hypoxia-induced acute oxidative stress injury to the stomach and small intestine.
关键词
槐花/外泌体/低氧/氧化应激/胃/小肠Key words
Sophora japonica L.flower/Exosome/Hypoxia/Oxidative stress/Stomach/Small bowel引用本文复制引用
基金项目
军事医学创新工程重点专项(18CXZ027)
出版年
2024
NETL
NSTL
万方数据