A study on lipidomic profiling of portal vein plasma in cirrhotic patients with clini-cally significant portal hypertension
Objective To elucidate the lipidomic profiles in the portal vein plasma of cirrhotic patients with clinically significant portal hypertension(CSPH).Methods A cohort of 26 cirrhotic patients from the First Medical Center of the Chinese PLA General Hospital from Feb.2021 to May 2023 was included in the study.Interventional procedures were employed to obtain portal vein plasma samples.Patients were stratified into CSPH group(HVPG≥10 mmHg)and non-CSPH group(HVPG<10 mmHg).Lipidomic analyses were conducted on the samples by the ultra high performance liq-uid chromatography/mass spectrometry(UPLC/MS)platform.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were applied as multivariate statistical approaches.Selection of differential lipid metabolites adhered to predefined criteria,including VIP>1,P<0.05,and FC>1.5 or<1/1.5.Results The CSPH group displayed significantly diminished levels of Hb,platelets and serum albumin in comparison to the non-CSPH group,and a notably higher Child-Pugh score than the non-CSPH group,demonstrating statistical signifi-cance(P<0.05).Extensive comparisons of demographic parameters,spanning gender,age,BMI and various clinical indicators beyond Hb,platelets and serum albumin revealed no statistically significant differences between the two groups(P>0.05).PCA and OPLS-DA analyses unveiled distinctive variations in lipid composition patterns between the two groups.A comprehensive identification of 48 differential lipid metabolites ensued,encompassing compounds such as carnitine,ceramide,lactosylceramide,lysophosphatidylethanolamine,phosphatidylcholine,phosphatidylethanolamine,phosphatidylglycerol and phosphatidylinositol.Conclusion The UPLC/MS platform can effectively screen differential lipid metabolites between patients with clinically significant portal hypertension in cirrhosis and those without clinically significant portal hypertension.This discovery provides a new perspective for understanding metabolic changes in the course of cirrhosis and offers a theoretical basis for exploring potential diagnostic and therapeutic targets.
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