Effects of miR-210 on vascular permeability,angiogenesis and radiation resistance of liver cancer VEC cells by regulating HDAC2 in hypoxic environments
Objective To investigate the effects of miR-210 on vascular permeability,angiogenesis and radiation re-sistance of liver cancer VEC cells by regulating histone deacetylase 2(HDAC2)in hypoxic environments.Methods VEC cells were cultured in hypoxic and normal environments,and the expression of miR-210 and HDAC2 in VEC cells was detected using RT-qPCR and Western blotting.VEC cells were cultured in hypoxic environment and randomly separated into control group,negative control group,miR-210 inhibitor group,HDAC2 knockdown group and miR-210 inhibitor+HDAC2 overexpression group.MTT method and Edu staining were applied to detect the proliferation of VEC cells in va-rious groups under hypoxic environment;Transwell chamber method and tubular formation experiment were applied to detect the vascular permeability and angiogenesis of VEC cells in each group;Western blotting and ELISA were applied to detect the expression and release of VEGF in VEC cells in each group.The viability of cells in each group was meas-ured using MTT method and their radiation resistance index was measured.Results Compared with normal cultured VEC cells,the expression of miR-210,HDAC2 mRNA and protein in VEC cells increased under hypoxic conditions(P＜0.05).Compared with the control group,the miR-210 inhibitor group and HDAC2 knockdown group showed a de-crease in HDAC2 mRNA and protein expression,cell viability,proliferation rate,permeability intensity,tubular length,VEGF protein expression,VEGF level in cell culture medium and radiation resistance index(P＜0.05),there was no obvious difference in all indicators of cells in the negative control group(P＞0.05).Compared with the miR-210 inhibi-tor group,the miR-210 inhibitor+HDAC2 overexpression group showed an increase in HDAC2 mRNA and protein ex-pression,cell viability,proliferation rate,permeability intensity,tubular length,VEGF protein expression,VEGF level in cell culture medium and radiation resistance index(P＜0.05).Conclusion Downregulation of miR-210 in hypoxic environments can inhibit the proliferation,vascular permeability,angiogenesis and radiation resistance of liver cancer VEC cells by reducing the expression of HDAC2.
Hypoxic environmentmiR-210HDAC2Liver cancer VEC cellsAngiogenesisRadiotherapy resistance
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